Antioxidant activity, total phenolic and flavonoid contents and analyses of active compounds in Stemona tuberose Lour crude extracts

Abstract

In this research, the total phenolic contents (TPC), total flavonoid contents (TFC), antioxidant activities and analyses of active compounds in Stemona?? tuberose Lour crude extracts including roots, stems and leaved were investigated. All crude samples were extracted from dried samples preparing from an oven-drying method (at 70??C). The TPC and TFC were measured by Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively, while antioxidant activities were assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. It was found that crude extracts from roots showed higher total phenolic and flavonoid contents and better antioxidant activity than those from stems and leaves. The analysis of active compounds such as phenolic and flavonoid compounds in crude extracts were carried out via a high performance liquid chromatography (HPLC) technique.?? The results showed that three active compounds including p-coumaric acid, feruilc acid and p-hydroxybenzoic acid were detected in all crude extracts. The p-hydroxybenzoic acid was major phenolic compound in all crude extracts. In addition, the types and amounts of active compounds in crude extract from roots were higher than those from stems and leaves.