Screening of Gamma Aminobutyric Acid (GABA) and Anti-oxidant Producing Bacillus Isolated from the Honey of Bees and Stingless Bees
Abstract
In this study, 22 isolates of Bacillus spp. isolated from honey and stingless honey samples were investigated the GABA production. The result showed three isolates, namely BPW-SB21, BPW-SB17, BPW-14 were able to produce a maximum amount of GABA of
985.59 ??0.4 ng/mL, 963.65 ??1.3 ng/mL and 962.51 ??1.1 ng/mL, respectively. In addition, antioxidant activity was examined by DPPH assay and the result demonstrated that BPW-SB2, BPW-SB21, BPW-SB1 were able to produce a maximum amount of anti-oxidation of 75.44 ??0.5%, 72.30 ??1.6%, 70.80 ??0.2%, respectively. The results suggest that the newly isolates of Bacillus from honey and stingless honey samples may be a promising bacterial candidate for use as a potential GABA producer with antioxidant activity.
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