Aeration Rate Effect in Xanthan Production by Xanthomonas campestris ATCC 13951 on Synthetic and Sugar Cane Medium

Abstract

Xanthan gum is a heteropolysaccharide produced by Xanthomonas campestrisATCC 13951 (TISTR 1100). This polysaccharide has been extensively used in manyindustries such as food, adhesive, ceramic, cosmetics, leather, pharmaceutical andpetrochemical. The aeration rate effects at 0, 5, 10 and 15 ppm on xanthan production wereevaluated in a batch fermentation. Both of synthetic and sugar cane medium were comparedin order to determine the optimal condition for xanthan production. Results indicated that the15 ppm aeration rate was suitable for both substrates, whereas the xanthan yield was higherin sugar cane medium than synthetic medium. The experiment showed that the maximumxanthan production was 117.3 g/L with 6.8 g/L maximum biomass concentration. Whenaccounting for the growth fermentation kinetics, xanthan gum was considered as a growthassociated product. Results showed that the growth rate in synthetic medium increased 3 and5 times as the aeration rate was increased by 2 and 3 times. The increment in the growth wasresulting in the greater rate of xanthan production. Data revealed that an increasing ofaeration rate from 2 and 3 times was influenced the rate of xanthan production by 3 and 3.5times. In the sugar cane medium, the growth rate multiplied from 2 to 4 times as 2 and 3times increasing in aeration rate. To the same amount of this aeration rate, the greater rates ofxanthan production were obtained by 3 and 3.5 times.