A simple strategy to fabricate electrochemical sensor based on nickel nanoparticles modified glassy carbon electrode for the determination of glucose in urine

Abstract

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A non-enzymatic electrochemical sensor has been successfully fabricated using nickel nanoparticles as catalyst. Nickel nanoparticles were electrochemically deposited on a glassy carbon electrode surface by amperometric technique. Nickel nanoparticles modified glassy carbon electrode exhibited high catalytic activity for oxidation of glucose. The morphology of nickel nanoparticles was characterized by scanning electron microscopy (SEM). Electrochemical behaviors of glucose were investigated by cyclic voltammetry and chronoamperometry in alkaline aqueous solution. At the optimum conditions, the calibration curve for glucose determination was linear in the range of 0.05 ??? 5.00 mM and 5.00 ??? 17.5 mM and a low detection limit (S/N = 3) of 36 ??M. Furthermore, the proposed sensor was successfully applied to analyze glucose level in human urine samples when compared with clinical laboratory measurements. The glucose sensor showed a strong potential as a sensitive non-enzymatic and may be used in the future for the clinical diagnostic tool.