Influence of Sodium Acetate on Stemona Alkaloid Production in Hydroponic Culture of Stemona curtisii Hook.f.

Abstract

To evaluate a potential method for the improvement of Stemona alkaloids production in S. curtisii, the influence of sodium acetate was studied in the modified hydroponic culture. Eight week???old S. curtisii plantlets from in vitro culture were used as plant materials. Sodium acetate at concentration of 0, 10, 20 and 50 mg/L were added into the culture medium. The samples of roots and culture medium in each treatment were collected on week 2 and week 4 after the elicitor addition and then analyzed for Stemona alkaloids production namely oxyprotostemonine, stemocurtisine and stemocurtisinol by high performance liquid chromatography. The HPLC result showed that the highest contents of total stemocurtisine (32.24 ??g/g DW) and stemocurtisinol (89.46 ??g/g DW) were detected in the treatment with 10 mg/L sodium acetate for 4 weeks, which were 1.8 and 1.4 fold significantly higher than the control, respectively. While, the treatment with 20 mg/L sodium acetate for 2 weeks had the highest content of total oxyprotostemonine, which was 233.65 ??g/g DW (1.4 fold higher than the control). From this research, it was demonstrated that sodium acetate had a positive effect on the enhancement of these Stemona alkaloids production.

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Keywords: Elicitor, Hydroponic culture, Sodium acetate, Stemona alkaloid, Stemona curtisii