Investigation of antioxidant activity of active compounds in ethyl acetate crude extract from stem of Paederia foetida Linn.

Abstract

This research presents the antioxidant activity of active compounds in ethyl acetate crude extract from the stem of Paederia foetida Linn.by using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Antioxidant activity of ethyl acetate crude was investigated and the percentage of inhibition was 74.72%, indicating a good antioxidant activity. The ethyl acetate crude was then isolated by a silica gel column chromatography method. It was found that one separated fraction showed high percentage (59.15%) in the inhibition. The functional group and structure of the compound in this fraction was then analyzed via Fourier Transform Infrared Spectroscopy (FT-IR), Nuclear Magnetic Resonance (NMR) spectroscopy and Thin Layer Chromatography (TLC). These evidences implied that the fraction containing active compounds possibly composed of the flavonoid derivatives having hydroxyl and methyl groups as the substituent groups.Keywords: 2,2-diphenyl-1-picrylhydrazyl free scavenging, antioxidant, Paederia foetidaLinn.