Tissue culture of Bulbophyllum affine Lindl.

Abstract

In vitro culture of 8 month-old seedling of Bulbophyllum affine Lindl. was investigated. Explants were cultured on various medium formulas; Vacin and Went (VW) (1949), ?? Murashige and Skoog (MS) (1962), Murashige and Skoog (1962) and Knudson C (Kc) (1946) supplemented with 150 ml/l coconut water, 100 g/l potato, 50g/l banana and 2.0 g/l activated charcoal for 12 weeks. The results showed that highest number of shoots (3.75 shoots per explant) could be obtained when cultured on VW medium while the highest shoot length (1.48 cm), root number (12.57 roots per explant) and root length (0.93 cm) were observed on Kc medium. In vitro shoot culture was also performed on VW medium with a combination of sucrose (5, 10 and 20 g/l) and coconut water (50, 100 and 150 ml/l) for 12 weeks. It was found that the highest number of shoots (2.65 shoots per explant) could obtain on the medium supplemented with 20 g/l sucrose and 150 ml/l coconut water while the highest shoot length (3.21 cm) could be observed on the medium supplemented with 10 g/l sucrose and 150 ml/l coconut water. However, the highest root number (17.81 roots per explant) was observed on the medium containing 20 g/l sucrose and 100 ml/l coconut water whereas the highest root length (2.13 cm) was performed on the medium with 20 g/l sucrose and 50 ml/l coconut water, respectively. Besides, in vitro shoots of Bulbophyllum affine Lindl. were cultured on the medium with different combinations of TDZ and 2,4-D at 0, 0.1, 0.5, 1.0 and 2.0 mg/l for 8 weeks. The results showed that the medium supplemented with a combinations of TDZ and 2,4-D at 1.6: 0.6, 6.5:0.6 and 1.6 mg/l gave the highest shoot number at 5.7, 5.6 and 5.5 shoots, respectively. In vitro young plantlets were transplanted and grew in different planting materials for 12 weeks. The results showed that the highest percentage of survival (93 %) could obtain when transplanted to grow in either vermiculite or dry fern with no significant different.

Keywords: Bulbophyllum affine Lindl., In vitro, hormones