Effect of cytokinins on In vitro shoot proliferation of Stemona tuberosa Lour.
Abstract
In vitro shoot culture of Stemona tuberosa Lour was conducted on modified Murashige
and Skoog (1962) medium with various types of cytokinins (2iP, BA, Kinetin, Zeatin and TDZ) at
different concentrations for 30 days. The results indicated that the medium supplemented with 1.0
mg/l TDZ, 5 and 10 mg/l BA induced the better number of new shoots; 3.1??0.52, 2.62??0.12 and
2.6??0.76 shoots per explant, respectively. Furthermore, an increase cytokinin concentration resulted in decreasing shoot number. The largest diameter of embryogenic callus (4.60??0.48 cm) could be
induced on the medium supplemented with 1.0 mg/l TDZ.
and Skoog (1962) medium with various types of cytokinins (2iP, BA, Kinetin, Zeatin and TDZ) at
different concentrations for 30 days. The results indicated that the medium supplemented with 1.0
mg/l TDZ, 5 and 10 mg/l BA induced the better number of new shoots; 3.1??0.52, 2.62??0.12 and
2.6??0.76 shoots per explant, respectively. Furthermore, an increase cytokinin concentration resulted in decreasing shoot number. The largest diameter of embryogenic callus (4.60??0.48 cm) could be
induced on the medium supplemented with 1.0 mg/l TDZ.
Keyword: Stemona tuberosa Lour, In vitro culture, cytokinins, regeneration
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